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Int J Clin Exp Pathol 1(2):124-133;2008

Original Article
Prognostic Significance of Flow Cytometric Immunophenotyping in Acute Myeloid

Brian A. Webber, Melissa M. Cushing and Shiyong Li

Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA

Received 2 Aug 2007; accepted and available online 1 January 2008

Abstract: The prognostic significance of flow cytometric immunophenotyping (FCI) in acute myeloid leukemia (AML) has been controversial. In
this study, we re-investigated the possible role of FCI in the prediction of AML relapse following standard chemotherapy. A total of 209 AML
cases with follow-up information were analyzed. Among those, 78 cases were in remission (M:F=44/34; mean age of 48.9 years) and 131 had
relapse (M:F=71/60; mean age of 51.3 years). The expression of CD34, HLA-DR or a combination of both was significantly different between
the remission and relapse groups for all AML as well as AML without t(15;17). None of the pammyeloid markers or their combinations analyzed
was found to correlate with treatment outcomes. Complex cytogenetic abnormalities were more likely associated with relapse group than with
remission group, but were not statistically significant after excluding AML with t(15;17). In conclusion, FCI is useful in predicting treatment
outcome and disease relapse in AML. (IJCEP708010).

Key Words: Flow cytometric immunophenotyping, acute myeloid leukemia, acute promyelocytic leukemia, chromosome translocation,
cytogenetics, prognosis

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Address all correspondence to: Shiyong Li, MD, PhD, Department of Pathology and Laboratory Medicine, Emory University Hospital F143D,
1364 Clifton Road NE, Atlanta, GA 30322. Tel: 404-712-5456; Fax: 404-712-4140; Email: