Original Article High Endogenous Avidin Binding Activity: An Inexpensive and Readily Available Marker for the Differential Diagnosis of Kidney Neoplasms
Kazunori Kanehira, Johnny Hu, Thomas Pier, Linda Sebree and Wei Huang
Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53792, USA; Department of Pathology and Laboratory Medicine, University of Wisconsin Hospital and Clinics, Madison WI 53792, USA
Received 21 Oct 2007; accepted with revision 5 Nov 2007; available online 1 January 2008
Abstract: It has been documented that some tissues, such as salivary gland, liver, cardiac and skeletal muscles and kidney, have high level endogenous biotin or endogenous avidin binding activity (EABA). Limited data is available on EABA in renal cell neoplasms. A tissue microarray (TMA) was constructed that included oncocytoma (n=30), chromophobe renal cell carcinoma (RCC) (n=18), clear cell RCC (n=45), clear cell RCC with granular/eosinophilic (G/E) features (n=19), papillary RCC (n=21), papillary RCC with G/E features (n=29) and benign renal tissues (n=31). The TMA slides were stained with or without biotin blocker and analyzed using the automated cellular imaging system (ACIS®). Without biotin blocker, a high positive rate of EABA was found in oncocytoma (56/60, 93%) and normal renal tubules (46/60, 77%). A moderate positive rate of EABA was found in clear cell and papillary RCCs with G/E features (13/39, 33% and 19/55, 35%, respectively). Chromophobe RCC and RCC without G/E features had essentially no EABA. With biotin blocker, benign renal tissue and clear cell RCC were negative for EABA; but a significant number of renal oncocytoma (29/60, 48%) and a few papillary RCC with G/E features (5/52, 10%) remained positive for EABA. In conclusion, high EABA may be used to differentiate oncocytoma from chromophobe RCC, and the staining results must be interpreted with caution when avidin-biotin detection system is used in diagnosing renal neoplasms. (IJCEP710005).
Address all correspondence to: Wei Huang, MD, Department of Pathology and Laboratory Medicine, University of Wisconsin, 2500 Overlook Terrace, Madison WI 53792. Tel: (608) 262-5787; Email: email@example.com