IJCEP Copyright © 2007-All rights reserved.
Int J Clin Exp Pathol 1(5): 435-439;2008

Original Article
High Endogenous Avidin Binding Activity: An Inexpensive and Readily Available Marker
for the Differential Diagnosis of Kidney Neoplasms

Kazunori Kanehira, Johnny Hu, Thomas Pier, Linda Sebree and Wei Huang

Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53792, USA; Department of Pathology and Laboratory
Medicine, University of Wisconsin Hospital and Clinics, Madison WI 53792, USA

Received 21 Oct 2007; accepted with revision 5 Nov 2007; available online 1 January 2008

Abstract: It has been documented that some tissues, such as salivary gland, liver, cardiac and skeletal muscles and kidney, have high level
endogenous biotin or endogenous avidin binding activity (EABA). Limited data is available on EABA in renal cell neoplasms. A tissue
microarray (TMA) was constructed that included oncocytoma (n=30), chromophobe renal cell carcinoma (RCC) (n=18), clear cell RCC (n=45),
clear cell RCC with granular/eosinophilic (G/E) features (n=19), papillary RCC (n=21), papillary RCC with G/E features (n=29) and benign renal
tissues (n=31). The TMA slides were stained with or without biotin blocker and analyzed using the automated cellular imaging system (ACIS®).
Without biotin blocker, a high positive rate of EABA was found in oncocytoma (56/60, 93%) and normal renal tubules (46/60, 77%). A moderate
positive rate of EABA was found in clear cell and papillary RCCs with G/E features (13/39, 33% and 19/55, 35%, respectively). Chromophobe
RCC and RCC without G/E features had essentially no EABA. With biotin blocker, benign renal tissue and clear cell RCC were negative for
EABA; but a significant number of renal oncocytoma (29/60, 48%) and a few papillary RCC with G/E features (5/52, 10%) remained positive for
EABA. In conclusion, high EABA may be used to differentiate oncocytoma from chromophobe RCC, and the staining results must be interpreted
with caution when avidin-biotin detection system is used in diagnosing renal neoplasms. (IJCEP710005).

Key Words: Oncocytoma, endogenous avidin binding activity (EABA), chromophobe renal cell carcinoma

Full Text  PDF

Address all correspondence to: Wei Huang, MD, Department of Pathology and Laboratory Medicine, University of Wisconsin, 2500 Overlook
Terrace, Madison WI 53792. Tel: (608) 262-5787; Email:
whuang23@wisc.edu