IJCEP Copyright © 2007-All rights reserved.
Int J Clin Exp Pathol 1(6), 475-488; 2008

Review Article
Laser Capture Microdissection in the Genomic and Proteomic Era: Targeting the Genetic
Basis of Cancer

Barbara Domaze, Gregory T. MacLennan, Antonio Lopez-Beltran, Rodolfo Montironi, and Liang Cheng

Departments of Pathology and Laboratory Medicine and Urology, Indiana University School of Medicine, Indianapolis, IN, USA; Department of
Pathology, Case Western Reserve University, Cleveland, OH, USA; Department of Pathology, Cordoba University, Cordoba, Spain and Institute
of Pathological Anatomy and Histopathology, School of Medicine, Polytechnic University of the Marche Region (Ancona), United Hospitals,
Ancona, Italy

Received 25 Feb 2008; accepted and available online 15 March 2008

Abstract: The advent of new technologies has enabled deeper insight into processes at subcellular levels, which will ultimately improve
diagnostic procedures and patient outcome. Thanks to cell enrichment methods, it is now possible to study cells in their native environment.
This has greatly contributed to a rapid growth in several areas, such as gene expression analysis, proteomics, and metabolonomics. Laser
capture microdissection (LCM) as a method of procuring subpopulations of cells under direct visual inspection is playing an important role in
these areas. This review provides an overview of existing LCM technology and its downstream applications in genomics, proteomics,
diagnostics and therapy. (IJCEP802002).

Key Words: Laser capture microdissection, genomics, proteomics, carcinogenesis, clonality, biomarker discovery, prognosis

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Address all correspondence to: Liang Cheng, MD, Department of Pathology and Laboratory Medicine, Indiana University School of Medicine,
350 West 11th Street, Clarian Pathology Laboratory Room 4010, Indianapolis, IN 46202, USA.  Telephone: 317-491-6442; Fax: 317-491-6419;