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Int J Clin Exp Pathol 2(2),176-181;2009

Original Article
The Effect of Different Doses of Cigarette Smoke in a Mouse Lung Tumor Model

Ludmilla Nadir Santiago, Juliana de Camargo Fenley, Lúcia Campanario Braga, José Antônio Cordeiro and Patrícia M. Cury

Department of Pathology and Forensic Medicine and Department of Epidemiology and Public Health, Laboratory of Experimental Pathology,
Departament of Pathology and Forensic Medicine, Faculdade de Medicina de São José do Rio Preto, FAMERP, Brazil

Received 8 June 2008; Accepted in revision 20 July 2008; Available online 25 August 2008

Abstract: Few studies have used Balb/c mice as an animal model for lung carcinogenesis. In this study, we investigated the effect of different
doses of cigarette smoking in the urethane-induced Balb/c mouse lung cancer model. After injection of 3mg/kg urethane intraperitoneally, the
mice were then exposed to tobacco smoke once or twice a day, five times a week, in a closed chamber. The animals were randomly divided
into four groups. The control group (G0) received urethane only. The experimental groups (G1, G2 and G3) received urethane and exposure to
the smoke of 3 cigarettes for 10 minutes once a day, 3 cigarettes for 10 minutes twice a day, and 6 cigarettes for 10 minutes twice a day,
respectively. The mice were sacrificed after 16 weeks of exposure, and the number of nodules and hyperplasia in the lungs was counted. The
results showed no statistically significant difference in the mean number of nodules and hyperplasia among the different groups, suggesting
that the Balb/c mice are not suitable to study the pathogenesis of tobacco smoking-induced tumor progression in the lungs.(IJCEP806004).

Key Words: Experimental carcinogenesis, lung cancer, Balb/c mice, tobacco smoking, urethane

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Address all correspondence to: Patrícia Maluf Cury, MD, PhD, Faculdade de Medicina de São José do Rio Preto, Av Brig. Faria Lima, 5416,
CEP 15090-000, São José do Rio Preto, SP, Brazil. Tel/Fax: +55 17 32015056; Email:
pmcury@famerp.br