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Int J Clin Exp Pathol 2010;3(2):139-146

Original Article
Biomarker profile in breast carcinomas presenting with bone metastasis

Olga L. Bohn, Irem Nasir, Adam Brufsky, George C Tseng, Rohit Bhargava, Kim MacManus, Mamatha Chivukula

Departments of Pathology, Medical Oncology, Magee-Women’s Hospital of University of Pittsburgh Medical Center, Pittsburgh, PA, USA;
Department of Biostatistics, University of Pittsburgh, Pittsburgh, PA

Received August 12, 2009, accepted October 18, 2009, available online November 10, 2009

Abstract: Bone is the most preferred site for metastatic dissemination in breast cancer. The purpose of this study was to examine the
expression of a set of antibodies that could serve as predictive biomarkers associated with breast cancer metastasis in a subset of sixteen
(16) breast cancer patients who developed bone metastasis.  The clinical and pathologic data were obtained, and tissue microarrays were
constructed. Tissue microarray slides were stained for TFF-1, CXRC4, MMP1, PTHrP, HER2, CD44, FGFR3 and IL-11. The expression rates
were compared between the metastatic breast cancer to bone (MBC-B) group and a group of sixty-four (64) primary breast cancer (PBC). The
results demonstrated that MBC-B group patients were more likely to be HER2 positive (P = 0.016). There was no significant difference on
estrogen receptor or progesterone receptor expression between MBC-B group and PBC group (P > 0.05). There was a high expression of
CXCR4, MMP-1, CD44, TFF-1, PTHrP, FGFR3 and IL-11, in both, PBC and MBC-B, and no significant differences between the groups were
identified. We found that tumors associated with bone metastasis tended to be larger than 2 cm. The high morbidity associated to metastatic
breast cancer prompts the identification of predictive biomarkers of relapse of breast tumors to categorize patients at high risk of bone
metastasis and serve as targeted therapy. (IJCEP908002).

Key words: Breast cancer, bone metastasis, immunohistochemistry, HER2, ER, TFF-1

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Address all correspondence to:
Mamatha Chivukula, MD
Department of Pathology
Magee-Women’s Hospital of University of Pittsburgh Medical Center
300 Halket Street, Pittsburgh, PA 15213-3180
Fax: 412-641-6169
E-mail:
mchivukula@mail.magee.edu