Original Article Immunohistochemical organization patterns of the follicular dendritic cells, myofibroblasts and macrophages in the human spleen--- New considerations on the pathological diagnosis of splenectomy pieces
Pablo Guisado Vasco, José Luis Villar Rodríguez, José Ibañez Martínez, Ricardo González Cámpora, Hugo Galera Davidson
University Hospital Ramón y Cajal. Colmenar Viejo Road, km. 9,100. Internal Medicine Department. Zip code: 28034, Madrid, Spain; Department of Pathology. University Hospital Virgen Macarena. Doctor Fedriani Avenue w/n. Zip Code: 41009. Seville, Spain.
Received August 20, 2009; accepted December 3, 2009; available online December 10, 2009
Abstract: There is reliable information about how changes in spleen histology are influenced by the relationship among B and T lymphocytes, macrophages, dendritic cells and myofibroblasts. Moreover, it can be applied in the day-by-day pathology laboratory. This work intends to elucidate morpho-functional aspects of relationships of these cells in the different spleen compartments, how they are influenced by pathological conditions and how basic immunohistochemical techniques could optimize the histopathological diagnosis. We analyzed the usefulness of the monoclonal antibodies CD45RO, CD20, CD21, CD35, CD68, caldesmon, the smooth muscle α-actin type 1 (SMA-1) in 91 specimens. Results: CD21+ CD35+ follicular dendritic cells were organized into three patterns in agreement with the immune condition of the lymphoid follicle. The dendritic cell population located in the periarteriolar lymphoid sheath keeps a uniform and homogeneous structure. Smooth muscle α-actin type 1+ and caldesmon+ myofibroblasts draw two double rings: marginal–perifollicular and germinal–marginal. The latter is closely related to T-cells. CD68+ red pulp macrophages had clear, linear configuration. The interruption of this CD68+ linear pattern in splenic marginal zone lymphoma cases could be a criterion to differentiate it from reactive hyperplasia. CD45RO, CD20, CD21, CD68 and SMA- 1 provide a basic and quality immunohistochemical battery for a better comprehension of the human spleen and could improve its histopathological diagnosis. (IJCEP908004).
Address all correspondence to: Pablo Guisado Vasco. MD, PhD University Hospital Ramón y Cajal. Colmenar Viejo Road, km. 9,100. Internal Medicine Department. Zip code: 28034. Madrid. E-mail: email@example.com