IJCEP Copyright © 2007-All rights reserved.
Int J Clin Exp Pathol 2010;3(2):189-202

Original Article
Immunohistochemical organization patterns of the follicular dendritic cells,
myofibroblasts and macrophages in the human spleen--- New considerations
on the pathological diagnosis of splenectomy pieces

Pablo Guisado Vasco, José Luis Villar Rodríguez, José Ibañez Martínez, Ricardo González Cámpora, Hugo Galera Davidson

University Hospital Ramón y Cajal. Colmenar Viejo Road, km. 9,100. Internal Medicine Department. Zip code: 28034, Madrid, Spain;
Department of Pathology. University Hospital Virgen Macarena. Doctor Fedriani Avenue w/n. Zip Code: 41009. Seville, Spain.

Received August 20, 2009; accepted December 3, 2009; available online December 10, 2009

Abstract: There is reliable information about how changes in spleen histology are influenced by the relationship among B and T lymphocytes,
macrophages, dendritic cells and myofibroblasts. Moreover, it can be applied in the day-by-day pathology laboratory. This work intends to
elucidate morpho-functional aspects of relationships of these cells in the different spleen compartments, how they are influenced by
pathological conditions and how basic immunohistochemical techniques could optimize the histopathological diagnosis. We analyzed the
usefulness of the monoclonal antibodies CD45RO, CD20, CD21, CD35, CD68, caldesmon, the smooth muscle α-actin type 1 (SMA-1) in 91
specimens. Results: CD21+ CD35+ follicular dendritic cells were organized into three patterns in agreement with the immune condition of the
lymphoid follicle. The dendritic cell population located in the periarteriolar lymphoid sheath keeps a uniform and homogeneous structure.
Smooth muscle α-actin type 1+ and caldesmon+ myofibroblasts draw two double rings: marginal–perifollicular and germinal–marginal. The
latter is closely related to T-cells. CD68+ red pulp macrophages had clear, linear configuration. The interruption of this CD68+ linear pattern in
splenic marginal zone lymphoma cases could be a criterion to differentiate it from reactive hyperplasia. CD45RO, CD20, CD21, CD68 and SMA-
1 provide a basic and quality immunohistochemical battery for a better comprehension of the human spleen and could improve its
histopathological diagnosis. (IJCEP908004).

Key words: Follicular dendritic cells, myofibroblasts, macrophages, spleen, immune system, immunohistochemical, histopathology

Full text PDF

Address all correspondence to:
Pablo Guisado Vasco. MD, PhD
University Hospital
Ramón y Cajal. Colmenar Viejo Road, km. 9,100.
Internal Medicine Department. Zip code: 28034.
Madrid. E-mail: