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Int J Clin Exp Pathol 2010;3(2):177-188

Original Article
Oxidative stress and gene expression of antioxidant enzymes in the
streptozotocin-induced diabetic rats under hyperbaric oxygen exposure

Tokio Matsunami, Yukita Sato, Takuya Sato, Satomi Ariga, Toshiko Shimomura, Masayoshi Yukawa

Laboratory of Biomedical Science, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa 252-8510,

Received November 17, 2009; accepted November , 2009; available online November , 2009

Abstract: Diabetes mellitus (DM) causes not only hyperglycemia but oxidative stress, resulting mainly enhanced production of mitochondrial
reactive oxygen species (ROS). Hyperbaric oxygen (HBO) treatments are applied various diseases including diabetic patients with unhealing
foot ulcers, however, and also increases the formation of ROS. Recently, it has been reported that oxidative stress worsens many pathological
conditions including DM and obesity suggesting possible changes in regulation of genes associated with the oxidative stress, however, effects
of HBO which could induce ROS on the gene expressions of oxidative stress parameters in DM animals are unknown. The purpose of this
study is to investigate the effect of HBO exposure on the gene expression of three important antioxidant enzymes, cytosolic superoxide
dismutase (Cu-Zn SOD), cytosolic glutathione peroxidase (GPx-1), and catalase (CAT) in DM rats, respectively. We used streptozotocin-induced
DM model rats and examined both mRNA expressions and the activities of these antioxidant enzymes in the liver, skeletal muscle, and
pancreas. The mRNA expressions of Cu-Zn SOD and CAT decreased significantly (p < 0.001), and GPx increased significantly (p < 0.001) in all
the studied organs of DM rats under HBO exposure compared to those from DM-induced rats not exposed to HBO. Similarly, activities of these
three enzymes changed in accordance with the mRNA levels. These results suggested that DM induction and HBO exposure might
synergistically affect antioxidant enzymes, resulting increase of oxidative stress state. Thus, HBO exposure seems to be an excellent model
system for investigating oxidative stress. (IJCEP911003).

Key words: Diabetes mellitus, hyperbaric oxygen, oxidative stress, superoxide dismutase, glutathione peroxidase, catalase

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Address all correspondence to:
Yukita Sato, PhD
Laboratory of Biomedical Science, Department of Veterinary Medicine,
College of Bioresource Sciences, Nihon University
Fujisawa 252-8510, Japan
Phone and Fax: +81-466-84-3445
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