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Int J Clin Exp Pathol 2010;3(3):288-302
Original Article
Culture of K562 human myeloid leukemia cells in presence of fibronectin expresses and
secretes MMP-9 in serum free culture medium
Anindita Dutta, Triparna Sen, Amitava Chatterjee
Department of Receptor Biology & Tumor Metastasis, Chittaranjan National Cancer Institute, 37, S P Mukherjee
Road, Kolkata, India.
Received January 25, 2010, accepted February 12, 2010, available online: February 18, 2010
Abstract: The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility,
gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study the
fibronectin-integrin mediated signalling and modulation of MMP expression. Our experimental findings demonstrate that exposure of K562
cells in serum free medium in presence of fibronectin up-regulates the expression of pro-MMP-9 within 2 hrs. Phosphorylation of focal
adhesion kinase (FAK), ERK, PI-3K and nuclear translocation of EGFR and NF-κB upon FN binding demonstrate possible involvement of
FAK/PI-3K/ERK signalling pathways in the fibronectin-integrin mediated up regulation of MMP-9 expression. (IJCEP1001010).
Key words: MMP-9, fibronectin, α5β1 integrin, NF-κB, FAK
Full text PDF
Address all correspondence to:
Amitava Chatterjee, PhD
Department of Receptor Biology & Tumor Metastasis
Chittaranjan National Cancer Institute
37, S P Mukherjee Road, Kolkata – 700 026, India.
Tel:+91-9830128320; FAX: +91-033-2475-7606.
Email: amitavachatterjee24@gmail.com
Original Article
Culture of K562 human myeloid leukemia cells in presence of fibronectin expresses and
secretes MMP-9 in serum free culture medium
Anindita Dutta, Triparna Sen, Amitava Chatterjee
Department of Receptor Biology & Tumor Metastasis, Chittaranjan National Cancer Institute, 37, S P Mukherjee
Road, Kolkata, India.
Received January 25, 2010, accepted February 12, 2010, available online: February 18, 2010
Abstract: The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility,
gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study the
fibronectin-integrin mediated signalling and modulation of MMP expression. Our experimental findings demonstrate that exposure of K562
cells in serum free medium in presence of fibronectin up-regulates the expression of pro-MMP-9 within 2 hrs. Phosphorylation of focal
adhesion kinase (FAK), ERK, PI-3K and nuclear translocation of EGFR and NF-κB upon FN binding demonstrate possible involvement of
FAK/PI-3K/ERK signalling pathways in the fibronectin-integrin mediated up regulation of MMP-9 expression. (IJCEP1001010).
Key words: MMP-9, fibronectin, α5β1 integrin, NF-κB, FAK
Full text PDF
Address all correspondence to:
Amitava Chatterjee, PhD
Department of Receptor Biology & Tumor Metastasis
Chittaranjan National Cancer Institute
37, S P Mukherjee Road, Kolkata – 700 026, India.
Tel:+91-9830128320; FAX: +91-033-2475-7606.
Email: amitavachatterjee24@gmail.com
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