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Int J Clin Exp Pathol 2013;6(2):230-241
Original Article
Intracerebral neural stem cell transplantation improved the auditory of mice with
presbycusis
Hongmiao Ren, Jichuan Chen, Yinan Wang, Shichang Zhang, Bo Zhang
Department of Otolaryngology Head and Neck Surgery, Institute of Surgery Research&Daping Hospital, Third
Military Medical University, Chongqing 400042, China; Institute of Otolaryngology of Chongqing; Key Laboratory
of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical
University, Chongqing, 400042, China.
Received September 20, 2012; Accepted November 27, 2012; Epub January 15, 2013; Published February 1,
2013
Abstract: Stem cell-based regenerative therapy is a potential cellular therapeutic strategy for patients with incurable
brain diseases. Embryonic neural stem cells (NSCs) represent an attractive cell source in regenerative medicine
strategies in the treatment of diseased brains. Here, we assess the capability of intracerebral embryonic NSCs
transplantation for C57BL/6J mice with presbycusis in vivo. Morphology analyses revealed that the neuronal rate of
apoptosis was lower in the aged group (10 months of age) but not in the young group (2 months of age) after NSCs
transplantation, while the electrophysiological data suggest that the Auditory Brain Stem Response (ABR) threshold
was significantly decreased in the aged group at 2 weeks and 3 weeks after transplantation. By contrast, there was
no difference in the aged group at 4 weeks post-transplantation or in the young group at any time post-transplantation.
Furthermore, immunofluorescence experiments showed that NSCs differentiated into neurons that engrafted
and migrated to the brain, even to sites of lesions. Together, our results demonstrate that NSCs transplantation
improve the auditory of C57BL/6J mice with presbycusis. (IJCEP1209017).
Keywords: Presbycusis, neural stem cells, transplantation, C57BL/6J mice, primary auditory cortex, apoptosis,
auditory brain stem response (ABR)
Address all correspondence to:
Dr. Jichuan Chen
Department of Otolaryngology Head and Neck Surgery
Institute of Surgery Research&Daping Hospital
Third Military Medical University
Chongqing 400042, China.
Tel: +86 23 68757771
E-mail: chenjcmd@sina.com
Original Article
Intracerebral neural stem cell transplantation improved the auditory of mice with
presbycusis
Hongmiao Ren, Jichuan Chen, Yinan Wang, Shichang Zhang, Bo Zhang
Department of Otolaryngology Head and Neck Surgery, Institute of Surgery Research&Daping Hospital, Third
Military Medical University, Chongqing 400042, China; Institute of Otolaryngology of Chongqing; Key Laboratory
of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical
University, Chongqing, 400042, China.
Received September 20, 2012; Accepted November 27, 2012; Epub January 15, 2013; Published February 1,
2013
Abstract: Stem cell-based regenerative therapy is a potential cellular therapeutic strategy for patients with incurable
brain diseases. Embryonic neural stem cells (NSCs) represent an attractive cell source in regenerative medicine
strategies in the treatment of diseased brains. Here, we assess the capability of intracerebral embryonic NSCs
transplantation for C57BL/6J mice with presbycusis in vivo. Morphology analyses revealed that the neuronal rate of
apoptosis was lower in the aged group (10 months of age) but not in the young group (2 months of age) after NSCs
transplantation, while the electrophysiological data suggest that the Auditory Brain Stem Response (ABR) threshold
was significantly decreased in the aged group at 2 weeks and 3 weeks after transplantation. By contrast, there was
no difference in the aged group at 4 weeks post-transplantation or in the young group at any time post-transplantation.
Furthermore, immunofluorescence experiments showed that NSCs differentiated into neurons that engrafted
and migrated to the brain, even to sites of lesions. Together, our results demonstrate that NSCs transplantation
improve the auditory of C57BL/6J mice with presbycusis. (IJCEP1209017).
Keywords: Presbycusis, neural stem cells, transplantation, C57BL/6J mice, primary auditory cortex, apoptosis,
auditory brain stem response (ABR)
Address all correspondence to:
Dr. Jichuan Chen
Department of Otolaryngology Head and Neck Surgery
Institute of Surgery Research&Daping Hospital
Third Military Medical University
Chongqing 400042, China.
Tel: +86 23 68757771
E-mail: chenjcmd@sina.com
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