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Int J Clin Exp Pathol 2013;6(2):249-257
Original Article
Loss of dystrophin staining in cardiomyocytes: a novel
method for detection early myocardial infarction
Satwat Hashmi, Suhail Al-Salam
Department of Pathology, Faculty of Medicine& Health Sciences, United Arab Emirates University, ALAIN PO Box
17666, UAE
Received November 11, 2012; Accepted December 12, 2012; Epub January 15, 2013; Published February 1,
2013
Abstract: Myocardial infarction (MI) is the most frequent diagnosis made in majority of sudden death cases subjected
to clinical and medicolegal autopsies. When sudden death occurs at a very early stage of MI, traditional
macroscopic examination, or histological stains cannot easily detect the myocardial changes. For this reason we
propose a new method for detecting MI at an early stage. Murine model of MI was used to induce MI through permanent
ligation of left anterior descending branch of left coronary artery. Five groups of C57B6/J mice were used
for inducing MI, which includes 20 minutes, 30 minutes, one hour, four hours and 24 hours post MI groups. One
naïve group and sham-operated groups were used as controls. There is loss of dystrophin membranous staining in
cardiac myocytes occurs as early as 20 minutes post myocardial infarction. This can be used as a novel method to
diagnose early myocardial infarction in post mortem cases where diagnosis is unclear. In conclusion, evaluation of
immunohistochemical expression of dystrophin represents a highly sensitive method for detecting early myocardial
infarction due to the loss of staining in the infarcted areas. Dystrophin immunostaining can also be used to assess
myocardial architecture. (IJCEP1211011).
Keywords: Heart, myocardium, ischemia, myocardial infarction, early detection, dystrophin
Address all correspondence to:
Dr. Suhail Al-Salam
Department of Pathology
Faculty of Medicine and Health Sciences
United Arab Emirates University
Alain PO box 17666, UAE.
E-mail: suhaila@uaeu.ac.ae
Original Article
Loss of dystrophin staining in cardiomyocytes: a novel
method for detection early myocardial infarction
Satwat Hashmi, Suhail Al-Salam
Department of Pathology, Faculty of Medicine& Health Sciences, United Arab Emirates University, ALAIN PO Box
17666, UAE
Received November 11, 2012; Accepted December 12, 2012; Epub January 15, 2013; Published February 1,
2013
Abstract: Myocardial infarction (MI) is the most frequent diagnosis made in majority of sudden death cases subjected
to clinical and medicolegal autopsies. When sudden death occurs at a very early stage of MI, traditional
macroscopic examination, or histological stains cannot easily detect the myocardial changes. For this reason we
propose a new method for detecting MI at an early stage. Murine model of MI was used to induce MI through permanent
ligation of left anterior descending branch of left coronary artery. Five groups of C57B6/J mice were used
for inducing MI, which includes 20 minutes, 30 minutes, one hour, four hours and 24 hours post MI groups. One
naïve group and sham-operated groups were used as controls. There is loss of dystrophin membranous staining in
cardiac myocytes occurs as early as 20 minutes post myocardial infarction. This can be used as a novel method to
diagnose early myocardial infarction in post mortem cases where diagnosis is unclear. In conclusion, evaluation of
immunohistochemical expression of dystrophin represents a highly sensitive method for detecting early myocardial
infarction due to the loss of staining in the infarcted areas. Dystrophin immunostaining can also be used to assess
myocardial architecture. (IJCEP1211011).
Keywords: Heart, myocardium, ischemia, myocardial infarction, early detection, dystrophin
Address all correspondence to:
Dr. Suhail Al-Salam
Department of Pathology
Faculty of Medicine and Health Sciences
United Arab Emirates University
Alain PO box 17666, UAE.
E-mail: suhaila@uaeu.ac.ae
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