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Int J Clin Exp Pathol 2(6),574-582;2009
Original Article
Comparison of Bcl-2, CD38 and ZAP-70 Expression in Chronic Lymphocytic Leukemia
Rachel L. Sargent, Fiona E. Craig and Steven H. Swerdlow
Department of Pathology, Division of Hematopathology, University of Pittsburgh School of Medicine, Pittsburgh, PA
Received 06 April 2009; Accepted in revision 26 May 2009; Available online 16 June 2009
Abstract: Chronic lymphocytic leukemia (CLL) was previously considered a uniform disease characterized by autonomous over-expression of
bcl-2. Recently the pathogenic role of bcl-2 has been questioned and attention has turned to prognostic subtypes of CLL differing in CD38 and
ZAP-70 expression. However, the relationship between bcl-2 and CD38 or ZAP-70 expression remains uncertain and was investigated using
flow cytometric immunophenotyping of 50 CLL specimens. CLL cells were consistently bcl-2 positive but varied in expression level: mean
fluorescence intensity (MFI) 45-152. Although there was no significant difference in bcl-2 expression between CD38 or ZAP-70 positive and
negative specimens, an inverse correlation was identified between percentage of CD38 positive B-cells and bcl-2 MFI when all (p<0.03,
r2=0.10) and peripheral blood (p<0.004, r2=0.27) samples were analyzed. While bcl-2 levels do not appear to be a major discriminator
between indolent and more aggressive subtypes of CLL, CD38 and bcl-2 expression appear to be interrelated. (IJCEP904003).
Key Words: bcl-2, CD38, chronic lymphocytic leukemia, ZAP-70
Full text PDF
Address all correspondence to: Fiona E. Craig, M.D., Department of Pathology, Division of Hematopathology, University of Pittsburgh School of
Medicine, UPMC-Presbyterian Hospital, Room G312, 200 Lothrop Street, Pittsburgh, PA 15213. Tel: 412-647-5191; Fax: 412-647-4008; Email:
craigfe@upmc.edu
Original Article
Comparison of Bcl-2, CD38 and ZAP-70 Expression in Chronic Lymphocytic Leukemia
Rachel L. Sargent, Fiona E. Craig and Steven H. Swerdlow
Department of Pathology, Division of Hematopathology, University of Pittsburgh School of Medicine, Pittsburgh, PA
Received 06 April 2009; Accepted in revision 26 May 2009; Available online 16 June 2009
Abstract: Chronic lymphocytic leukemia (CLL) was previously considered a uniform disease characterized by autonomous over-expression of
bcl-2. Recently the pathogenic role of bcl-2 has been questioned and attention has turned to prognostic subtypes of CLL differing in CD38 and
ZAP-70 expression. However, the relationship between bcl-2 and CD38 or ZAP-70 expression remains uncertain and was investigated using
flow cytometric immunophenotyping of 50 CLL specimens. CLL cells were consistently bcl-2 positive but varied in expression level: mean
fluorescence intensity (MFI) 45-152. Although there was no significant difference in bcl-2 expression between CD38 or ZAP-70 positive and
negative specimens, an inverse correlation was identified between percentage of CD38 positive B-cells and bcl-2 MFI when all (p<0.03,
r2=0.10) and peripheral blood (p<0.004, r2=0.27) samples were analyzed. While bcl-2 levels do not appear to be a major discriminator
between indolent and more aggressive subtypes of CLL, CD38 and bcl-2 expression appear to be interrelated. (IJCEP904003).
Key Words: bcl-2, CD38, chronic lymphocytic leukemia, ZAP-70
Full text PDF
Address all correspondence to: Fiona E. Craig, M.D., Department of Pathology, Division of Hematopathology, University of Pittsburgh School of
Medicine, UPMC-Presbyterian Hospital, Room G312, 200 Lothrop Street, Pittsburgh, PA 15213. Tel: 412-647-5191; Fax: 412-647-4008; Email:
craigfe@upmc.edu
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